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After exposure, the cells were washed once in PBS and stained with JC-1 and propidium iodide (5 g/ml, Thermo Fisher) according to the protocol in serum free L-15 cell culture medium for 20 min at 19 C

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Within this intricate web of events, oxidative stress markers (OSMs) such as malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), and serum-free thiols (R-SH)emerge as valuable diagnostic targets for IBD diagnosis