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Dual degradation mechanism of GPX4 degrader in induction of ferroptosis exerting anti-resistant tumor effect

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The amplitude (A) of spontaneous and Ca 2+ -induced swelling of the organelle suspension in a Ca 2+ -free medium and in the conditions of pore-formation activation with the action of PTP inducer of swelling of calcium ions was calculated as the difference in optical density values from 1 to 15 min mPTP-associated mitochondrial swelling was tested by the specific mPTP inhibitor cyclosporin A (CsA), which was added to the incubation medium in a concentration of 10 5 mol/L 5 min before the induction of pore formation

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Behavioral assessments (open-field, self-grooming, social interactions) were followed by biochemical and molecular analyses in the cerebellum, where oxidative stress markers (MDA, GSH, SOD, CAT, and GPx) were quantified spectrophotometrically, and Nrf2, Bax, Bcl-2, and Caspase-3 mRNA levels were measured by q RT-PCR