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Glutathione is a master antioxidant that helps the body manage oxidative stress

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Life Technologies, CA) to yield total RNA following the manufacturers instructions

In the next, extremely unfavorable elimination step (step 2), the conjugate base of the amine is kicked out from the tetrahedral intermediate: The reaction is driven to completion in a quick, irreversible deprotonation of the carboxylic acid by the newly formed RNH

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