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Oxidative stress was quantified through ROS, nitrites, TBARS, total thiols, and the activities of SOD, CAT GPx, GST, and ALA-D, using spectrophotometric assays and fluorescence-based detection

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Note that while elevated homocysteine is associated with an increased risk of Alzheimer's disease, systematic reviews of clinical trials comparing vitamin B12 supplementation to placebo groups have not shown a significant effect in preventing or slowing Alzheimer's disease or cognitive decline

warm slightly in hand if needed, but dont heat externally

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