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Co-expression network reveals the potential relationship between glutathione with DEGs and DEMs In addition to core genes and metabolites of glutathione metabolism KEGG pathway, 6 DEGs ( cgs1 , 100381533 , bHLH11 , AR7 , OHP2 , MatE, abc1 ) and 5 DEMs (1,3-Benzodioxol-5-yl(oxo)acetic acid, dihydroplumbagin, 6-(2-methoxy-Z-vinyl)-7-methyl-pyranocoumarin, (7R)-(+)-Lyoniresinol 9-glucoside, and Capsanthin-3,6-epoxide) derived from co-expression network those were potentially involved in glutathione metabolism

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This involved perfusing the brains with phosphate-buffered saline followed by 4% paraformaldehyde, sectioning them into eight-micrometer coronal slices, and visually confirming the cannulas location within the left lateral ventricle [1]

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