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They were split into three groups

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Consuming too few calories without medical supervision can lead to nutritional deficiencies, muscle loss, fatigue, and metabolic adaptation that may impair long-term weight management

PMID 1741380

If left at room temperature for 8-12 hours, there will likely be some degradation, but it may not be a total loss

His team of doctors and health experts are constantly monitoring everything his body does, from his cholesterol levels to the length of his telomeres DNA caps that prevent our chromosomes from fraying, like the plastic tips on shoelaces, and are thought to play a key role in cell death and ageing